Journal: STAR Protocols
Article Title: Covalent anchoring of tethered ligands to chemogenetic handles for targeted in vivo neuropharmacology
doi: 10.1016/j.xpro.2025.104186
Figure Lengend Snippet: In vivo acute electrophysiological recordings with CATCH (A) Stereotaxic frame equipped with a Thomas Recording (TRec) Matrix, enabling precise injection and recording with multiple independently movable tetrodes. (B) Application of fluorescent dyes to the tip of a glass micropipette (or injection cannula) using a P2 pipette. This allows for post-hoc verification of the glass micropipette track to confirm accurate targeting of the brain region. (C) Catheterization of the saphenous vein for i.v. drug administration, such as nicotine. A needle tip attached to a plastic tube is inserted into the vein close to an 90° angle to avoid perforation. Adhesive tape on the paw and pipette tips placed behind the hind limb help to tense the leg, facilitating easier access to the saphenous vein. (D) Lowering the tetrodes into the brain following craniotomy and removal of the dura mater.
Article Snippet: PIPETMAN P2 micropipette , Gilson , Cat#F144801.
Techniques: In Vivo, Injection, Transferring, Adhesive